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This tool can compare DNA and RNA sequences in various formats, including FASTA and plain text. You can directly upload sequence files or fetch sequences from NCBI using their accession IDs.
The tool performs a pairwise alignment between your reference and query sequences to identify differences. It categorizes mutations as substitutions (base changes), insertions (added bases), or deletions (removed bases). The algorithm also calculates statistics like mutation rate and distribution across different regions.
Yes, you can filter results by mutation type (substitution, insertion, deletion) and by specific positions or regions of interest. This helps you focus on the most relevant changes for your research.
The PDF report includes comprehensive analysis details: summary statistics, sequence metadata, a visualization of mutation distribution, a complete table of all mutations with their positions and types, and citation information for academic use.
You can cite this tool using standard academic formats (APA, MLA, Chicago) with the DOI: 10.5281/zenodo.15069907. Citation formats are provided at the bottom of the results page and included in the generated PDF reports.
The tool is optimized for sequences up to 1 million base pairs. Larger sequences can be processed but may take longer. For very large genomic sequences (>10 million bp), we recommend using specialized desktop software.